We will understand the reasons behind the formation of various precipitation patterns in this assay. In early work, immunodiffusion tests were carried out in small tubes i. Immunodiffusion in gels encompasses a variety of techniques, which are useful for the analysis. Ouchterlony double diffusion for antigenantibody patterns amrita. In this study the ouchterlony double immunodiffusion. Use of the ouchterlony double immunodiffusion method to. However, this type of test has been superseded by double diffusion tests in two dimensions on glass slides. Travaux pratiques dimmunologie licence des sciences. Ouchterlony double immunodiffusion also known as passive double immunodiffusion is an immunological technique used in the detection, identification and quantification of antibodies and antigens, such as immunoglobulins and extractable nuclear antigens. These notes correspond to the white keys of the piano.
Ouchterlony double diffusion for antibody titration amrita. Geneitm ouchterlony double diffusion teaching kit manual ispybio. Synopsis introduction historical background types of immunodiffusion principle ouchterlony. To learn the technique of ouchterlony double diffusion. In this video lecture, we will understand in detail the double diffusion assay. Ouchterlony double diffusion odd or double immunodiffusion technique is one of the simplest techniques extensively used to check antisera. Radial immunodiffusion can quantitatively determine the concentration of an antibody. This video channel is developed by amrita universitys create amrita. Interaction between antigen ag and antibody ab at the molecular level forms the basis for. Double immunodiffusion ouchterlony double diffusion fl. For more information, visit this video demonstrates the ouchterlony double immunodiffusion.
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