The order of these notes is c, d, e, f, g, a, and b. Immunodiffusion in gels encompasses a variety of techniques, which are useful for the analysis. Interaction between antigen ag and antibody ab at the molecular level forms the basis for. Ouchterlony double diffusion for antigenantibody patterns amrita. In this study the ouchterlony double immunodiffusion. Ouchterlony double diffusion odd or double immunodiffusion technique is one of the simplest techniques extensively used to check antisera. This video channel is developed by amrita universitys create amrita. The key reaction of immunology and immune defense is the interaction of antibodies.
Use of the ouchterlony double immunodiffusion method to. Synopsis introduction historical background types of immunodiffusion principle ouchterlony. Double immunodiffusion ouchterlony double diffusion fl. Ouchterlony double immunodiffusion also known as passive double immunodiffusion is an immunological technique used in the detection, identification and quantification of antibodies and antigens, such as immunoglobulins and extractable nuclear antigens. Radial immunodiffusion can quantitatively determine the concentration of an antibody. We will understand the reasons behind the formation of various precipitation patterns in this assay. Travaux pratiques dimmunologie licence des sciences. In this video lecture, we will understand in detail the double diffusion assay. These notes correspond to the white keys of the piano.
In early work, immunodiffusion tests were carried out in small tubes i. For more information, visit this video demonstrates the ouchterlony double immunodiffusion. To learn the technique of ouchterlony double diffusion. Immunodiffusion techniques applications to determine relative concentrations of antibodies antigens. Ouchterlony double diffusion for antibody titration amrita. Geneitm ouchterlony double diffusion teaching kit manual ispybio.
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